the co-effect of deuterium depleted water and satureja rechingeri essential oils on drug metabolizing enzyme activities in liver of rats treated with acetaminophen

abstract this study was carried out to investigate the hepatoprotective effect of deuterium depleted water with satureja rechingeri essential oil on liver damage. for this purpose, the adult male rats were divided into 16 groups. the negative control group used tap water in 14 days following dmso i.p injection at day 15th. the treatment group with acetaminophen received tap water in 14 days following 500mg/kg b.w. i.p injection of acetaminophen dissolved in 300ml dmso at day 15th. the treatment groups receiving deuterium depleted water (30ppm and 60 ppm) plus i.p injection of satureja rechingeri essential oil following 500 mg/kg b.w. acetaminophen injection at day 15th.then, at 4,8,16 and 24 hours after acetaminophen injection, the blood was taken from hurt puncture and the liver tissues were removed. then, the biochemical marker levels such as ast, alt, alp and bilirubin were measured in plasma. the glutathione s-transferase enzyme activity and protein level, cytochrome p450 protein level and glutathione level were also estimated in liver homogenate. findings indicated no significant changes in the level of serum parameters such as alt, alp and bilirubin in groups. while injecting acetaminophen decreased the levels of gst and gsh and increased significantly the level of cyp450 and ast. treatment with deuterium depleted water and satureja rechingeri essential oils are effective in moderating the levels of these parameters, leaded to returning of these parameters to normal values. finally, the treatment of rats with high dose of acetaminophen caused liver oxidative damage and using of deuterium depleted water and satureja rechingeri essential oil can be effective in preventing and improving these damages.